Plant Transcription Factor Database
Previous version: v3.0
Solanum pimpinellifolium
FAR1 Family
Species TF ID Description
Sopim00g012700.0.1FAR1 family protein
Sopim01g096850.0.1FAR1 family protein
Sopim01g103180.0.1FAR1 family protein
Sopim01g112320.0.1FAR1 family protein
Sopim02g068550.0.1FAR1 family protein
Sopim02g092170.0.1FAR1 family protein
Sopim03g113110.0.1FAR1 family protein
Sopim03g117520.0.1FAR1 family protein
Sopim03g121630.0.1FAR1 family protein
Sopim05g014930.0.1FAR1 family protein
Sopim06g054040.0.1FAR1 family protein
Sopim06g068200.0.1FAR1 family protein
Sopim06g068210.0.1FAR1 family protein
Sopim06g073200.0.1FAR1 family protein
Sopim06g073210.0.1FAR1 family protein
Sopim06g073670.0.1FAR1 family protein
Sopim07g043260.0.1FAR1 family protein
Sopim07g043270.0.1FAR1 family protein
Sopim07g045520.0.1FAR1 family protein
Sopim08g060840.0.1FAR1 family protein
Sopim08g076330.0.1FAR1 family protein
Sopim09g057870.0.1FAR1 family protein
Sopim09g057880.0.1FAR1 family protein
Sopim09g075640.0.1FAR1 family protein
Sopim10g084220.0.1FAR1 family protein
Sopim11g017210.0.1FAR1 family protein
Sopim11g051000.0.1FAR1 family protein
FAR1 Family Introduction

We show that Arabidopsis FHY3 and FAR1, which encode two proteins related to Mutator-like transposases, act together to modulate phyA signaling by directly activating the transcription of FHY1 and FHL, whose products are essential for light-induced phyA nuclear accumulation and subsequent light responses. FHY3 and FAR1 have separable DNA binding and transcriptional activation domains that are highly conserved in Mutator-like transposases. Further, expression of FHY3 and FAR1 is negatively regulated by phyA signaling. We propose that FHY3 and FAR1 represent transcription factors that have been co-opted from an ancient Mutator-like transposase(s) to modulate phyA-signaling homeostasis in higher plants.

We next used a yeast one-hybrid assay to delineate the DNA sequences to which FHY3 and FAR1 bind. GAD-FHY3 or GAD-FAR1 fusion proteins (GAD, GAL4 transcriptional activation domain), but not GAD alone, activated the LacZ reporter genes driven by the FHY1 and FHL promoters. Deletion analysis narrowed down the FHY3/FAR1 binding site to a 39-bp promoter subfragment located on the "a" fragment for both FHY1 and FHL. Notably, these subfragments share a stretch of consensus sequence, 5'-TTCACGCGCC-3'. Mutating the core sequence "CACGCGC" of this motif (m2 and m3 for FHY1, m5 for FHL) abolished the reporter gene activation by both GAD-FHY3 and GAD-FAR1. Mutating the flanking sequences (m1 and m4) did not obviously affect the reporter gene activation by GAD-FAR1, but clearly reduced activation by GAD-FHY3. Thus, "CACGCGC" likely defines a cis-element that confers specific binding for FHY3 and FAR1 and is named FBS for FHY3-FAR1 binding site.

Lin R, Ding L, Casola C, Ripoll DR, Feschotte C, Wang H.
Transposase-derived transcription factors regulate light signaling in Arabidopsis.
Science, 2007. 318(5854): p. 1302-5.
PMID: 18033885